Biopac Student Lab                   Lesson 7: Blood Pressure, ECG & Pulse


ECG & Pulse

Mechanical Action of the Heart, Peripheral Pressure Pulse, Plethysmography, and blood pressure



Student’s Name: ______________________________________


Lab Section: ___________________________________________


Date. _____________________________________________



I.  Data and Calculations

 Subject Profile 

Name _____________________________


Height ________________________


Age ______________________________


Weight ________________________

Gender:            Male or Female


A.     Comparison of ECG with Pulse Plethysmogram (Segments 1-3) Complete Table 7.1 with data from three cycles from each segment and calculate the Means.

Table 7.1




Cycle 1

Cycle 2

Cycle 3


At rest

Segment 1

R-R Interval

One cardiac cycle

DT      CH 1





Heart Rate

Pulse rate

BPM   CH 1





Blood pressure systolic/diastolic

Mm Hg












Immediately after exercise

Segment 2

R-R Interval

One cardiac cycle

DT      CH 1





Heart Rate

Pulse Rate

BPM   CH 1





Blood pressure systolic/diastolic

mm Hg












Temperature change (hand in cold water)

Segment 3

R-R Interval

One cardiac cycle

DT      CH 1





Heart Rate

Pulse Rate

BPM   CH 1





Blood pressure systolic/diastolic

mm Hg






B.    Relative Volume Changes (Segments 1-3)

Complete Table 7.2 with data from each recording segment.


Table 7.2 

Measurements (average of three cycles)
At Rest
Segment 1
After Exercise

Segment 2

Hand in cold H2O
Segment 3

QRS Amplitude

CH1 p-p




Relative Pulse Amplitude


CH 2 p-p




QT interval (corresponds to ventricular systole)





End of T wave to subsequent R wave (correspond to ventricular diastole)





C.    Calculation of Pulse Speed

               Distance between Subject’s sternum and shoulder? ________________          cm   

              Distance between Subject’s shoulder and fingertip? ________________          cm

              Total distance? ________________   cm


 Segment 1 Data (use I-beam to select data from Segment 1 of the recording)


Time between R-wave and Pulse peak? ______________________seconds

              Speed?            cm/sec

              Segment 2 Data (use I-beam to select data from Segment 2 of the recording)

Time between R-wave and Pulse peak? ______________________secs

              Speed?              cm/sec
II.           Questions

A.    Referring to data in table 7.1, are the values of heart rate and pulse rate similar for each condition? Yes/No Explain why the values might differ or be similar.




B.   How does the ratio between the durations of ventricular systole and ventricular diastole change from resting to exercise state?  (Table 7.2)




C.   How does the amplitude of QRS and relative pulse amplitude change from resting to exercise, to having hand in cold water?  (Table 7.2)






D.  How is blood pressure affected by exercise and having hand in cold water?







E.    Describe one mechanism that causes changes in blood volume to your fingertip.







F.   Referring to data from section C of this report, how would you explain the difference in speed, if any?








G.  Which components of the cardiac cycle (atrial systole and diastole, ventricular systole and diastole) are discernible in the pulse tracing?









H. Would you expect the calculated pulse wave velocities of other students to be very close if not the same as yours? Why or why not?









Computerized Blood Pressure Monitoring Protocol


  1. Double click “Omron” IC3
  2. Attach computer cable to monitor
  3. Attach power cord to monitor, turn blood pressure monitor on
  4. Login:  Dr_Group, OK


Create a “measurable schedule”


  1. Go to File
  2. Select New Patient, àenter patient ID
  3. Enter your name
  4. Enter name—first, middle, and last
  5. Enter ‘sex’ of subject
  6. Tab to “System schedule”
  7. Enter time range
  8. Optional-- Select “Measurement alarm”
  9.  Enter time range—be sure to use military time
  10. Select interval 10 minutes, OK
  11. Select “Add”
  12. Send schedule to monitor
  13. Go to Tool, select “schedule and send”
  14. Select name
  15. Select “open” on right lower corner
  16. Question-- delete old data, select “yes”
  17. Transmit
  18. Disconnect computer cable from monitor
  19.  Connect cuff to monitor
  20. Turn “on” blood pressure monitor
  21. Put cuff on your upper arm
  22. Push “start” on monitor
  23. Take blood pressure
  24.  Time intervals of blood pressure measurement as indicated by instructor
  25. Push “start” for each additional measurement


Download stored information:

  1. Turn monitor off
  2. Plug computer cord to monitor
  3. Turn on monitor
  4. Go to tool, select “ get data from device”
  5. Select OK


View received data:

  1. Blood pressure monitor reading screen File
  2. Click OK  (patient name)
  3. Click “yes”


Select display format:

  1. Go to view
  2.  View options:  table of data, histogram, and/or statistic
  3. View “statistic” to see data—obtain highs, lows, and averages
  4. Click on each to see details
  5. Change scale if graph is too small
  6. To increase the scale- X axis-- Go to view, select vertical axis
  7. Select horizontal axis, select “Custom”, choose 1 hour


To test the change in blood pressure under separate events,

e.g. place hand in cold water, after exercise… etc

  1. Place hand in cold water/after exercise
  2. Press “event”
  3. Press “start”
  4. Measure blood pressure 3X
  5. Measure recovery blood pressure


Be sure to unplug cords and return the blood pressure cuff and monitor to the box given to you by your instructor.



End of Lesson 7 Data Report